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KMID : 0880220120500060987
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Journal of Microbiology
2012 Volume.50 No. 6 p.987 ~ p.993
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A new quorum-sensing inhibitor attenuates virulence and decreases antibiotic resistance in Pseudomonas aeruginosa
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Yu-Xiang Yang
Zhen-Hua Xu
Yu-Qian Zhang
Li-Xing Weng
Jing Tian
Lian-Hui Wang
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Abstract
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Quorum sensing (QS) has been a novel target for the treatment of infectious diseases. Here structural analogs of Pseudomonas aeruginosa autoinducer N-acyl homoserine lactone (AHL) were investigated for QS inhibitor (QSI) activity and a novel QSI was discovered, N-decanoyl-L-homoserine benzyl ester (C2). Virulence assays showed that C2 down-regulated total protease and elastase activities, as well as the production of rhamnolipid, that are controlled by QS in P. aeruginosa wild-type strain PAO1 without affecting growth. C2 was also shown to inhibit swarming motility of PAO1. Using a microdilution checkerboard method, we identified synergistic interactions between C2 and several antibiotics, tobramycin, gentamycin, cefepime, and meropenem. Data from real-time RT-PCR suggested that C2 inhibited the expression of lasR (29.67%), lasI (21.57%), rhlR (28.20%), and rhlI (29.03%).
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KEYWORD
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Pseudomonas aeruginosa, quorum sensing, inhibitor, virulence factor, swarming, antimicrobial susceptibilit
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